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Solid-phase derivatization reactions for biomedical liquid chromatography

I S Krull1, M E Szulc, A J Bourque

  • 1Department of Chemistry, Northeastern University, Boston, MA 02115.

Journal of Chromatography. B, Biomedical Applications
|September 23, 1994
PubMed
Summary
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Polymeric reagents enable off- and on-line derivatization for High-Performance Liquid Chromatography (HPLC). These advanced materials enhance analyte detection with improved sample cleanup and quantitation in complex biofluids.

Area of Science:

  • Analytical Chemistry
  • Polymer Science
  • Chromatography

Background:

  • Development of polymeric reagents for derivatization in High-Performance Liquid Chromatography (HPLC).
  • Utilizes ionic or covalent attachment of detector-enhancing tags (UV, electrochemical, fluorescence).
  • Involves specific synthetic procedures for tag attachment to polymeric supports via linkages like activated esters.

Purpose of the Study:

  • To review the evolution and applications of polymeric reagents in HPLC and capillary electrophoresis.
  • To highlight the engineering of polymer supports, attachment leashes, and tags for analyte derivatization.
  • To discuss the commercialization and practical applicability of these reagents for complex biofluid samples.

Main Methods:

  • Design and synthesis of polymeric reagents with specific tag functionalities.

Related Experiment Videos

  • Application of polymeric reagents for off- and on-line derivatization of organic analytes.
  • Integration of polymeric reagents into workflows including solid-phase extraction, HPLC, and multiple detection modes.

    • Main Results:

    • Polymer properties (hydrophobicity, pore size) influence reaction efficiency and tag stability.
    • Engineered polymeric reagents allow for direct injection of biofluids with enhanced sample cleanup.
    • Successful application in real-world drug and bioorganic analysis, enabling accurate quantitation.

    Conclusions:

    • Polymeric reagents represent a significant advancement in chromatographic analysis, offering versatile derivatization strategies.
    • These reagents facilitate streamlined workflows, from sample preparation to final data interpretation.
    • The technology is commercially available and practically applicable for analyzing complex biological samples.