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Derivatization of thiol-containing compounds

K Shimada1, K Mitamura

  • 1Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.

Journal of Chromatography. B, Biomedical Applications
|September 23, 1994
PubMed
Summary

This review covers thiol derivatization reagents for analyzing biological fluids. It details reagents

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Area of Science:

  • Biochemistry
  • Clinical Chemistry

Background:

  • Thiol-containing compounds are crucial in biological systems.
  • Accurate determination of thiols in biological fluids is vital for biochemical and clinical applications.

Purpose of the Study:

  • To review derivatization reagents for thiols.
  • To assess reagents based on reactivity, selectivity, and spectroscopic properties.
  • To highlight applicability in various analytical techniques, especially high-performance liquid chromatography (HPLC).

Main Methods:

  • Review of literature on thiol derivatization reagents.
  • Analysis of functional groups (e.g., N-substituted maleimide, active halogen, aziridine) that react with thiols.
  • Discussion of derivatization for ultraviolet and electrochemical detection.

Main Results:

  • Various derivatization reagents exhibit different reactivity and selectivity profiles.
  • Reagents are suitable for diverse detection methods including UV and electrochemical detection.
  • Applicability extends to techniques like flow injection analysis, thin-layer chromatography, and gas chromatography-mass spectrometry.

Conclusions:

  • Effective derivatization is key for accurate thiol quantification in biological samples.
  • The choice of reagent depends on the analytical method and desired specificity.
  • This review provides a comprehensive overview for selecting appropriate thiol derivatization strategies.

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