Related Experiment Videos
Oxidative stress in haemodialysis
C M Loughrey1, I S Young, J H Lightbody
1Regional Nephrology Unit, Belfast City Hospital, UK.
QJM : Monthly Journal of the Association of Physicians
|November 1, 1994
Summary
Chronic renal failure and hemodialysis increase oxidative stress, indicated by higher lipid peroxidation and lower antioxidant levels. This oxidative stress may contribute to cardiovascular disease in these patients.
Area of Science:
- Nephrology
- Cardiovascular Medicine
- Biochemistry
Background:
- Patients with chronic renal failure (CRF) exhibit a high rate of premature cardiovascular disease.
- Oxidative stress is a proposed contributor to atherogenesis, the development of atherosclerosis.
Purpose of the Study:
- To investigate whether chronic renal failure and hemodialysis are associated with increased oxidative stress.
- To assess markers of lipid peroxidation and antioxidant status in patients with CRF and those undergoing hemodialysis.
Main Methods:
- Serum malondialdehyde (MDA) was measured as a marker of lipid peroxidation.
- Levels of selenium, glutathione peroxidase, and antioxidant vitamins (including vitamin C) were quantified.
- Three groups were studied: 15 conservatively managed CRF patients, 15 hemodialysis patients, and 15 healthy controls.
Main Results:
- Malondialdehyde levels were significantly elevated in hemodialysis patients compared to both CRF patients and controls.
- Antioxidant levels (vitamin C, selenium, glutathione peroxidase) were decreased in hemodialysis patients and, to a lesser extent, in CRF patients compared to controls.
- These findings demonstrate increased lipid peroxidation and reduced antioxidant capacity in patients with CRF, particularly those undergoing hemodialysis.
Conclusions:
- Chronic renal failure is associated with oxidative stress, evidenced by increased lipid peroxidation and diminished antioxidant levels.
- Hemodialysis further exacerbates oxidative stress in patients with chronic renal failure.
- Oxidative stress likely plays a significant role in the accelerated atherosclerosis observed in patients with chronic renal failure and those on hemodialysis.