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Related Experiment Videos

Apoptosis during macrophage-dependent ocular tissue remodelling

R Lang1, M Lustig, F Francois

  • 1Skirball Institute for Biomolecular Medicine, Developmental Biology and Genetics, New York University Medical Center, NY 10016.

Development (Cambridge, England)
|December 1, 1994
PubMed
Summary

The pupillary membrane

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Area of Science:

  • Ophthalmology
  • Developmental Biology
  • Cell Biology

Background:

  • The pupillary membrane is a transient vascular network in the anterior eye chamber.
  • It comprises capillaries, endothelial cells, extracellular matrix, and macrophages.
  • Understanding its regression is key to ocular development.

Purpose of the Study:

  • To characterize cell death during pupillary membrane regression.
  • To elucidate the pattern and mechanism of capillary regression.
  • To determine the role of macrophages in this process.

Main Methods:

  • Morphological analysis of pupillary membrane regression.
  • Identification of apoptotic bodies and condensed chromatin.
  • TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) assay for fragmented DNA.
  • Genomic DNA fragmentation analysis.

Main Results:

  • Cell death during regression is primarily apoptosis.
  • Apoptosis occurs in single cells or synchronously along capillary segments.
  • Capillary regression proceeds segment by segment.
  • Macrophages induce apoptosis in target cells.

Conclusions:

  • Pupillary membrane regression involves macrophage-induced apoptosis.
  • Capillary regression follows a specific junction-to-junction pattern.
  • This study provides a model for understanding vascular regression during development.

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