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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
Published on: April 18, 2016
Characterization and functional activity of dendritic cells from rat choroid
A Choudhury1, V A Pakalnis, W E Bowers
1Department of Microbiology and Immunology, University of South Carolina School of Medicine, Columbia 29208.
Experimental Eye Research
|September 1, 1994
Summary
Functional dendritic cells (DC) were identified in the posterior eye cup, specifically within the choroid. These cells play a role in immune responses and may be involved in posterior uveitis.
Area of Science:
- Ophthalmology
- Immunology
- Cell Biology
Background:
- Dendritic cells (DC) are crucial immune cells involved in T-cell responses.
- The presence and function of DC in the posterior eye compartment are not well-established.
Purpose of the Study:
- To investigate the presence and function of dendritic cells (DC) in the posterior eye cup.
- To characterize the localization and potential role of these cells in ocular immune responses.
Main Methods:
- Cell culture of posterior eye cup preparations.
- Assays for T-cell responses and mixed leukocyte reactions (MLR).
- Immunomagnetic separation and immunofluorescent staining using anti-DC antibodies.
- Cryostat sectioning and histological analysis of eye tissue.
Main Results:
- Eye cup cell cultures exhibited accessory T-cell activity and MLR stimulatory capacity, characteristic of DC.
- These activities were associated with low-density cells.
- Immunoselection identified a distinct subpopulation of low-density cells with DC morphology and surface antigens.
- These cells were localized to the choroid, distinct from the sclera and retina.
Conclusions:
- Functional dendritic cells (DC) are present in the choroid of the eye.
- These choroidal DC may play a significant role in the inflammatory processes of posterior uveitis.
