Related Experiment Videos
Calpains in lens epithelium from patients with cataract
M Andersson1, J Sjöstrand, A K Andersson
1Department of Ophthalmology, University of Göteborg, Sweden.
Experimental Eye Research
|September 1, 1994
Abstract:
Lens epithelium from patients with cataract was obtained during surgery and frozen. The samples were subjected to SDS-electrophoresis and Western blotting. Calpains were quantified using polyclonal antibodies against m- and mu-Calpain could be detected but not the isoenzyme mu-calpain, indicating that m-calpain is the significant most important calpain in human lens epithelium. Quantification of m-calpain showed no relationship to age or gender, but there were significant differences between different types of cataract.