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Sequence and chromosomal localisation of the gene encoding ovine latent transforming growth factor-beta 1
C J Woodall1, L J McLaren, N J Watt
1Department of Veterinary Pathology, Summerhall, University of Edinburgh, UK.
Gene
|December 15, 1994
Abstract:
Transforming growth factor-beta 1 (TGF-beta 1) plays important roles in pathologic processes. To further investigate the actions of this cytokine in sheep, the entire 1170-bp ovine TGF-beta 1 pro-protein-encoding sequence has been determined by the cloning and sequencing of specific polymerase-chain-reaction amplification products of TGF-beta 1 cDNA sequences. In addition, these sequences have been used to estimate the length of the TGF-beta 1 mRNA as 1.5-1.7 kb by Northern blot hybridization and determine that the ovine TGF-beta 1 gene occupies a single locus in the sheep genome by chromosomal in situ hybridization.