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Memory B lymphocytes in human tonsil do not express surface IgD

I C Nicholson1, M J Brisco, H Zola

  • 1Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, Australia.

Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1995
PubMed
Summary
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Memory B lymphocytes, crucial for adaptive immunity, were analyzed for somatic hypermutations. Results show memory B cells are primarily surface IgD-negative, indicating distinct B cell phenotypes.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Memory B lymphocytes are essential for long-term immunity.
  • The expression of immunoglobulin D (IgD) on memory B cells remains a debated topic in immunology.

Purpose of the Study:

  • To investigate the phenotype of memory B lymphocytes.
  • To determine the frequency of somatic hypermutations in different tonsil B cell populations.
  • To clarify whether memory B cells express surface IgD (sIgD).

Main Methods:

  • Purification of tonsil B cell populations.
  • Amplification, cloning, and sequencing of Ig heavy chain gene rearrangements using the VH6 gene.
  • Analysis of somatic hypermutation frequency in sIgD-negative and sIgD-positive fractions.

Main Results:

Related Experiment Videos

  • Somatic hypermutations were frequent in the sIgD-negative B cell fraction (1 mutation/24 bases).
  • Somatic hypermutations were infrequent in the sIgD-positive B cell fraction (1 mutation/600 bases), similar to naive cord blood B cells.
  • Memory B cells were detected in the sIgD-negative fraction, but not in the sIgD-positive fraction.

Conclusions:

  • Memory B cells are predominantly found within the sIgD-negative population of tonsil B lymphocytes.
  • The sIgD-positive fraction of tonsil B cells does not appear to contain memory B cells.
  • This study clarifies the phenotype of memory B cells regarding IgD expression.