Incubation temperature as a modifying factor on survival of Tenebrio molitor reared in selenium-containing media

Newly emerged Tenebrio molitor were reared at 4, 25, and 37 degrees C in nutrient media supplemented with sodium selenate (0.0125, 0.0200, 0.0500, and 0.1000%). Ten insects comprised each group. Controls were maintained in unsupplemented medium at the same temperatures as the experimental groups. Survival percentages were determined. Survival curves were estimated at given times postincubation. Controls at 4 and 37 degrees C showed an increased lethality compared to those insects at 25 degrees C. Data indicate that 4 degrees C had a protective effect on survival for insects reared in media containing the three highest concentrations of selenium. For insects at 37 degrees C, killing was striking and equivalent for all groups, irrespective of media supplementation.