Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Beta-lactamase activity in Shigella sonnei

A Prince, H C Neu

    Antimicrobial Agents and Chemotherapy
    |May 1, 1976
    PubMed
    Summary

    Ampicillin resistance in Shigella sonnei is complex, involving multiple beta-lactamase enzymes. These enzymes vary among strains, impacting antibiotic effectiveness and treatment strategies for Shigella infections.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Regarding: "reconstructive and salvage techniques for proximal interphalangeal joint fracture-dislocations".

    Journal of hand and microsurgery·2026
    Same author

    Postpartum care: Discussions and counseling for the peripartum period.

    Journal of neonatal-perinatal medicine·2023
    Same author

    Practices of sickle cell disease genetic screening and testing in the prenatal population.

    Journal of neonatal-perinatal medicine·2022
    Same author

    Sun-dried Azolla leaf meal at 10% dietary inclusion improved growth, meat quality, and increased skeletal muscle Ribosomal protein S6 kinase β1 abundance in growing rabbit.

    Animal : an international journal of animal bioscience·2021
    Same author

    Influence of level of inclusion of Azolla leaf meal on growth performance, meat quality and skeletal muscle p70S6 kinase α abundance in broiler chickens.

    Animal : an international journal of animal bioscience·2020
    Same author

    Reduction of Prolonged Excessive Pressure in Seated Persons With Paraplegia Using Wireless Lingual Tactile Feedback: A Randomized Controlled Trial.

    IEEE journal of translational engineering in health and medicine·2018

    Area of Science:

    • Microbiology
    • Bacterial genetics
    • Antimicrobial resistance

    Background:

    • Shigella sonnei is a significant cause of bacterial dysentery.
    • Ampicillin resistance in bacteria is often mediated by beta-lactamase enzymes.
    • Understanding resistance mechanisms is crucial for effective treatment.

    Purpose of the Study:

    • To investigate the molecular basis of ampicillin resistance in Shigella sonnei isolates.
    • To characterize the beta-lactamase enzymes involved in ampicillin resistance.
    • To determine the diversity of resistance mechanisms within Shigella sonnei.

    Main Methods:

    • Analysis of ampicillin-resistant Shigella sonnei isolates.
    • Testing inhibition by various beta-lactam antibiotics.
    • Assessing beta-lactamase activity and enzyme profiles using acrylamide electrophoresis.
    • Evaluating antibiotic hydrolysis and resistance transfer.

    Main Results:

    • Ampicillin resistance in Shigella sonnei is not solely due to transferable resistance.
    • Multiple distinct beta-lactamase enzymes mediate ampicillin resistance.
    • No direct correlation was found between antibiotic inhibitory concentration and hydrolysis rate.
    • Isolates can possess more than one type of beta-lactamase.

    Conclusions:

    • Ampicillin resistance in Shigella sonnei is multifactorial, involving diverse beta-lactamases.
    • The presence of multiple beta-lactamases complicates resistance prediction and treatment.
    • Further research into specific beta-lactamase characterization is warranted for targeted therapies.

    Related Experiment Videos