Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single-day apolipoprotein E genotyping

R Crook1, J Hardy, K Duff

  • 1Suncoast Alzheimer's Disease Laboratories, Department of Psychiatry, University of South Florida, Tampa 33613.

Journal of Neuroscience Methods
|August 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of temperature and aggregation rate on the fractal dimension of renneted casein aggregates.

Journal of dairy science·2003
Same author

Inconsistent designs of association studies: a missed opportunity.

Molecular psychiatry·2003
Same author

'Tag and label' system for checking and recording of blood transfusions.

Transfusion medicine (Oxford, England)·2003
Same author

LR-90 a new advanced glycation endproduct inhibitor prevents progression of diabetic nephropathy in streptozotocin-diabetic rats.

Diabetologia·2003
Same author

Parkin-proven disease: common founders but divergent phenotypes.

Neurology·2003
Same author

Evidence for insect transmission of rabbit haemorrhagic disease virus.

Epidemiology and infection·2003
Same journal

Corrigendum to "Human peripheral nerve xenografts and rat peripheral nerve allografts implanted to the striatum: Methodology and initial findings of cell-based therapy" [J. Neurosci. Methods 434 (2026) 110825].

Journal of neuroscience methods·2026
Same journal

Sombor-based graph-theoretic framework for the structural characterization of neuro-metabolic organic acids.

Journal of neuroscience methods·2026
Same journal

Pupil-DLC: an open-source deep learning pipeline for scalable, marker-less tracking of pupil dynamics across conscious and unconscious states.

Journal of neuroscience methods·2026
Same journal

Time as the language of Behavior: events, sequences, patterns and meanings.

Journal of neuroscience methods·2026
Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026
Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026
See all related articles

Genotyping apolipoprotein E (ApoE) alleles is currently slow. Using agarose gel electrophoresis offers a faster and simpler method for separating DNA fragments, improving ApoE genotyping efficiency.

Area of Science:

  • Biochemistry
  • Genetics
  • Molecular Biology

Background:

  • Apolipoprotein E (ApoE) genotyping is crucial for understanding genetic predispositions to various diseases.
  • Current ApoE genotyping methods rely on polyacrylamide gel electrophoresis, which is labor-intensive and time-consuming.

Purpose of the Study:

  • To evaluate the efficacy of agarose gel electrophoresis as a faster alternative for ApoE genotyping.
  • To simplify the process of separating digested DNA fragments for ApoE allele determination.

Main Methods:

  • DNA extraction and digestion.
  • Separation of digested DNA fragments using agarose gel electrophoresis.
  • Comparison of results with traditional polyacrylamide gel electrophoresis methods.

Related Experiment Videos

Main Results:

  • Agarose gel electrophoresis allows for quicker separation of digested ApoE DNA fragments compared to polyacrylamide gels.
  • The method provides clear and easily interpretable results for ApoE genotyping.
  • The procedure is less complex and more accessible than current standard techniques.

Conclusions:

  • Agarose gel electrophoresis is a viable and advantageous method for ApoE genotyping.
  • This technique offers a more efficient and user-friendly approach for clinical and research settings.
  • Adoption of agarose gel electrophoresis can significantly reduce the time and resources required for ApoE genotyping.