Protection from hypoxemic renal dysfunction by verapamil and manganese in the rabbit

The ability of calcium channel blockers to prevent and/or to reverse the hypoxemia-induced renal dysfunction was studied in anesthetized and mechanically-ventilated rabbits. Renal blood flow (RBF) and glomerular filtration rate (GFR) were determined by para-aminohippuric acid and inulin clearance, respectively. Each animal was considered as its own control. In 9 rabbits (group 1), verapamil infusion, 1 microgram.kg-1.min-1, did not change basal renal hemodynamics. In a second group (n = 9), hypoxemia induced marked significant decreases in GFR, RBF and urine flow rate (-22 +/- 5%, -18 +/- 6% and -34 +/- 7% respectively). The administration of verapamil partially reversed the hypoxemia-induced renal dysfunction. Likewise, hypoxemia did not induce any change in renal function in verapamil pretreated rabbits (n = 8). This study shows that verapamil is able to prevent and to partially reverse the adverse effects of hypoxemia on renal hemodynamics. The intrarenal infusion of manganese chloride, another calcium channel blocker, was also effective in blunting the hypoxemia-induced changes in the left infused kidney, but not in the contralateral kidney. The overall results demonstrate that a significant protection from hypoxemic renal dysfunction can be achieved by inhibitors of calcium ion transport.