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SV40 virus expression vectors

H Y Naim1, M G Roth

  • 1Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235.

Methods in Cell Biology
|January 1, 1994
PubMed
Summary
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Simian virus 40 (SV40) late-replacement vectors enable high protein expression for molecular biology research. These vectors are ideal for studying protein mutants and performing sensitive detection techniques.

Area of Science:

  • Molecular Biology
  • Virology
  • Protein Expression Systems

Background:

  • SV40 late-replacement vectors are utilized for expressing proteins from cDNA.
  • Efficient protein production is crucial for various molecular biology applications.

Purpose of the Study:

  • To highlight the utility of SV40 late-replacement vectors for protein expression.
  • To demonstrate their suitability for specific experimental techniques and mutant analysis.

Main Methods:

  • Utilizing SV40 late-replacement vectors for cDNA cloning and protein expression.
  • Employing techniques such as pulse-chase protocols and immunocytochemistry on cryosections.

Main Results:

  • SV40 vectors facilitate the expression of large protein quantities from cDNAs < 2400 bp.

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  • Sufficient protein levels are achieved 28 hours post-infection for brief pulse-chase studies.
  • Protein yield is adequate for sensitive detection methods like immunocytochemistry in healthy cells.

    • Conclusions:

    • SV40 late-replacement vectors are effective tools for high-level protein expression.
    • Their ease of use, reproducibility, and suitability for sensitive assays make them valuable for comparing site-directed mutagenesis mutants.