Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peptide sequences binding to MHC class I proteins

M H Smith1, K S Lam, E M Hersh

  • 1Department of Biochemistry, University of Arizona, Tucson 85721.

Molecular Immunology
|December 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A randomized, controlled phase III trial of nab-Paclitaxel versus dacarbazine in chemotherapy-naïve patients with metastatic melanoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2015
Same author

Transduction of human dendritic cells with a recombinant modified vaccinia Ankara virus encoding MUC1 and IL-2.

Cancer immunology, immunotherapy : CII·2001
Same author

Cationic lipid-mediated gene transfer: current concepts.

Current opinion in molecular therapeutics·2001
Same author

Phase II study of direct intralesional gene transfer of allovectin-7, an HLA-B7/beta2-microglobulin DNA-liposome complex, in patients with metastatic melanoma.

Clinical cancer research : an official journal of the American Association for Cancer Research·2001
Same author

Preclinical antitumor activity of the azonafide series of anthracene-based DNA intercalators.

Anti-cancer drugs·2001
Same author

A phase I and pharmacodynamic evaluation of polyethylene glycol-conjugated L-asparaginase in patients with advanced solid tumors.

Cancer chemotherapy and pharmacology·2001
Same journal

m6A modification of LINC00458 enhances HMOX1 stability via ELAVL1 recruitment to promote ferroptosis and aggravate asthma.

Molecular immunology·2026
Same journal

Overexpression of Hes1 inhibits cigarette smoke-induced mitochondrial apoptosis in AT2 cells by activating the Pgc-1α/Tfam signaling pathway.

Molecular immunology·2026
Same journal

Progesterone promotes favorable pregnancy outcomes in recurrent spontaneous, abortion by attenuating NK Cell overactivation and upregulating the cAMP/PKA/CREB signaling axis.

Molecular immunology·2026
Same journal

Oleanolic acid alleviates hepatic fibrosis by inhibiting liver macrophage recruitment and polarization.

Molecular immunology·2026
Same journal

Cordycepin attenuates diabetic nephropathy by dual-pathway activation of TFEB to restore autophagy and ameliorate podocyte injury.

Molecular immunology·2026
Same journal

Endothelial-derived TWEAK drives granulosa cell apoptosis in PCOS via the Fn14-oxidative stress axis.

Molecular immunology·2026
See all related articles

Researchers identified specific peptide binding motifs for HLA-A2 and HLA-B7 molecules using a synthetic peptide library. This method efficiently reveals allele-specific binding patterns, comparable to analyzing naturally occurring peptides.

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Major Histocompatibility Complex (MHC) class I molecules present peptides to T cells.
  • Understanding peptide-MHC binding is crucial for immunology and vaccine development.
  • Allele-specific binding motifs guide the identification of T cell epitopes.

Purpose of the Study:

  • To determine allele-specific peptide binding motifs for human MHC class I molecules HLA-A2 and HLA-B7.
  • To evaluate the utility of a random synthetic peptide library for identifying these motifs.
  • To compare this method with traditional approaches using endogenous peptides.

Main Methods:

  • Sequence analysis of nonamer peptides selected from a random synthetic library after binding to HLA-A2 and HLA-B7.

Related Experiment Videos

  • Utilizing denatured class I heavy chains for peptide selection.
  • Comparing results from synthetic libraries with those from endogenous peptides.

    • Main Results:

    • Identified four critical peptide positions for HLA-A2 binding and three for HLA-B7 binding.
    • Demonstrated that synthetic peptide libraries can effectively identify allele-specific binding motifs.
    • Achieved results comparable to sequencing endogenous peptides.

    Conclusions:

    • A synthetic peptide library approach is a viable method for determining MHC class I binding motifs.
    • This method provides insights into peptide-MHC interactions, valuable for predicting or engineering immunomodulatory T cell epitopes.
    • The technique is adaptable, working with purified or expressed class I proteins.