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Pan-neuronal mRNA expression of the secretory polypeptide 7B2
M Marcinkiewicz1, P Touraine, M Chrétien
1J.A. de Sève Laboratory of Molecular Neuroendocrinology, Clinical Research Institute of Montréal (affiliated to the University of Montréal), Que., Canada.
Abstract:
The polypeptide 7B2 exhibits a widespread distribution in the CNS and in the endocrine tissues. By in situ hybridization in the mouse tissues, we detected 7B2-mRNA transcripts in most, if not all, neurons of the brain and spinal cord, and in the cranial and spinal ganglia. 7B2-mRNA was undetectable in supportive glial cells, ependymal cells and endothelial cells. In embryonic tissues, 7B2-mRNA expression was observed at midgestation, starting on day 11. Both differentiated neurons and neuronal precursors have been shown to express 7B2 transcript. We conclude that 7B2-mRNA is a good molecular marker of developing and definitive neurons.