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Characterization of a G14 equine rotavirus (strain CH3) isolated in Japan
M Takagi1, K Taniguchi, T Urasawa
1Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Archives of Virology
|January 1, 1994
Abstract:
Antigenic and genomic properties of equine rotavirus strain CH3 isolated in Japan were studied by cross-neutralization tests and nucleotide sequence determination of the VP4 and VP7 genes. It was shown that the strain CH3 belongs to G14 and shares VP4 genotype with strain H2.
Insights
Equine rotavirus strain CH3 from Japan was analyzed. This strain belongs to G14 and shares a VP4 genotype with strain H2, providing insights into equine rotavirus diversity.
Area of Science:
- Veterinary Virology
- Molecular Epidemiology
Background:
- Equine rotaviruses (ERVs) are significant enteric pathogens in foals.
- Understanding the genetic diversity of ERVs is crucial for disease control and vaccine development.
Purpose of the Study:
- To characterize the antigenic and genomic properties of a novel equine rotavirus strain, CH3, isolated in Japan.
- To determine the G and P genotypes of the CH3 strain.
Main Methods:
- Cross-neutralization tests were performed to assess antigenic properties.
- Nucleotide sequence determination of the VP4 and VP7 genes was conducted for genomic analysis.
Main Results:
- Equine rotavirus strain CH3 was identified as G14 based on VP7 gene analysis.
- The VP4 gene of strain CH3 indicated a shared genotype with the H2 strain.
Conclusions:
- Equine rotavirus strain CH3 represents a G14P[unknown] strain, contributing to the known G-genotype diversity in Japanese equine rotaviruses.
- The genetic characterization of strain CH3 aids in understanding the epidemiology and evolution of equine rotaviruses.