Related Experiment Video
Updated: Oct 2, 2026

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
Published on: October 28, 2014
Cytosolic calcium is essential in the basal expression of tyrosine hydroxylase gene
1Department of Life Science, Pohang University of Science and Technology, Republic of Korea.
Abstract:
To test the role of cytosolic calcium in the basal expression of a neuronal gene, promoter activity of the rat tyrosine hydroxylase (TH) gene was monitored upon reduced resting level of intracellular calcium. TH promoter activity was decreased by cell-permeable calcium chelator, BAPTA/AM, in SK-N-BE(2)C human neuroblastoma cells. The cAMP response element (CRE) was mapped to the calcium responsible element by mutational and deletional analysis of the 5' upstream promoter region. Gel shift assay showed 2 CRE-specific DNA-protein complexes. The quantities of specific complexes were markedly decreased in BAPTA/AM-treated cells. These data suggest that resting level of intracellular calcium has a critical role in the basal expression of TH gene through the regulation of the binding of nuclear proteins to the CRE motif in the promoter.
More Related Videos
00:09Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
Published on: February 18, 2020
08:01Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
Published on: January 19, 2024
Related Concept Videos
Cell Specific Gene Expression
Circadian Rhythms and Gene Regulation
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Synthesis and Functions of Calcitonin
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...