Multinuclear magnetic resonance studies of boar seminal plasma

Multinuclear magnetic resonance studies were performed on aqueous solutions of lyophilisates of boar seminal plasma. 1H- and 13C-NMR spectral assignments were obtained by one- and two-dimensional experiments. Four prominent constituents were identified in the lyophilisate as well as in the original seminal plasma: inositol (95% myo-inositol, 5% scyllo-inositol), citrate, lactate and glycerophosphorylcholine (GPC). The concentrations of these compounds were evaluated from appropriate 1H- and 13C-NMR resonances using biochemically determined citrate as reference. 31P-NMR spectroscopy confirmed the presence of GPC and revealed phosphorylcholine, glycerophosphorylserine and glycerophosphorylethanolamine as further components.