Related Experiment Video

Updated: Aug 11, 2026

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
07:17

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

Role of substrains in persistent lymphocytic choriomeningitis virus infection

J Hotchin, W Kinch, L Benson

    Bulletin of the World Health Organization
    |January 1, 1975
    PubMed

    Insights

    LCM virus variants exhibit distinct behaviors in cell cultures. Some variants persist, producing virus cyclically, while others cease virus production, highlighting differences in LCM virus persistence.

    Area of Science:

    • Virology
    • Cell Biology
    • Immunology

    Background:

    • Lymphocytic choriomeningitis virus (LCMV) can establish persistent infections.
    • Understanding LCMV variants is crucial for studying viral persistence and pathogenesis.

    Purpose of the Study:

    • To compare the behavior of different LCMV strains and variants in cell culture.
    • To investigate the relationship between virus production, plaque formation, and immunofluorescence in persistent infections.

    Main Methods:

    • Infection of L cell cultures with plaque-type mutants, a wild strain (MB(1)), and a slow-growing non-plaque-forming LCMV variant.
    • Monitoring of plaque-forming virus titers and specific immunofluorescence (cytoplasmic and membrane) at biweekly intervals.
    • Serial transfer of infected cultures over multiple passages.

    Main Results:

    • The LM variant continuously produced virus for 35 transfers with cycling titers.
    • The SC variant ceased detectable virus production after 30 transfers but remained fluorescent, similar to the slow-growing variant.
    • The MB(1) strain stopped virus production after 18 transfers.
    • Specific immunofluorescence continued to cycle in cultures infected with the LM and slow-growing variants.

    Conclusions:

    • Different LCMV strains and variants exhibit distinct patterns of viral replication and persistence in cell culture.
    • Viral persistence is associated with continued cellular immunofluorescence, even after virus production ceases.
    • The study provides insights into the mechanisms underlying LCMV persistence and the heterogeneity of viral behavior.

    More Related Videos

    Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors06:02

    Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

    Published on: September 13, 2018

    Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice05:03

    Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice

    Published on: April 26, 2024

    Related Concept Videos

    Subviral Agents01:29

    Subviral Agents

    Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
    Inhibitors of Viral Protein Synthesis01:30

    Inhibitors of Viral Protein Synthesis

    Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
    Inhibitors Of Virion Release01:25

    Inhibitors Of Virion Release

    Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
    Inhibitors of Virion Maturation and Assembly01:19

    Inhibitors of Virion Maturation and Assembly

    As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...