Cell specific expression of a vasopressin transgene in rats

A transgene consisting of the rat vasopressin structural gene containing a reporter in exon III, flanked by 5 kb of upstream and 3 kb of downstream sequences, is expressed in vasopressinergic, but not oxytocinergic, magnocellular neurons of rats. Functionally appropriate physiological stimuli increase transgene expression in magnocellular neurons in an exaggerated fashion; the magnitude of the transgene response to osmotic challenge exceeds that of the endogenous gene by 10-15 fold. Magnocellular vasopressinergic neurons in the rat are now accessible to rational and precise genetic perturbation of function and regulation.