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[Fiberoptic bronchoscope disinfection with glutaraldehyde phenolate in 1:8 solution]
G Rodríguez-Froján1, J Castella, C Puzo
1Departamento de Neumología, Hospital de la Santa Creu i Sant Pau, Barcelona.
Archivos De Bronconeumologia
|December 1, 1994
Summary
Phenolated glutaraldehyde effectively disinfects bronchofibroscopes contaminated with Serratia marcescens and Pseudomonas aeruginosa. A 1:8 solution, after washing, achieved complete disinfection within 10 minutes.
Area of Science:
- Microbiology
- Infectious Diseases
- Medical Device Disinfection
Background:
- Bronchofibroscopes are critical for diagnosing respiratory conditions.
- High contamination levels of bacteria like Serratia marcescens and Pseudomonas aeruginosa pose infection risks.
- Effective disinfection protocols are essential to prevent healthcare-associated infections.
Purpose of the Study:
- To evaluate the efficacy of phenolated glutaraldehyde (1:8 solution) for disinfecting bronchofibroscopes.
- To determine the disinfection time required for highly contaminated instruments.
- To assess the effectiveness against Serratia marcescens and Pseudomonas aeruginosa.
Main Methods:
- Bronchofibroscopes were artificially contaminated with high concentrations (approx. 10(8) cfu/ml) of S. marcescens or P. aeruginosa.
- Instruments underwent washing with soap and water.
- Submersion in a 1:8 phenolated glutaraldehyde solution for 10, 15, or 30 minutes.
- Microbial cultures were performed post-contamination, post-washing, and post-disinfection.
Main Results:
- Pre-disinfection washing significantly reduced microbial load.
- No microbial growth (failure-to-disinfect) was observed after 10 minutes of submersion in the 1:8 phenolated glutaraldehyde solution.
- The disinfection protocol was effective against both tested microorganisms.
Conclusions:
- A 1:8 solution of phenolated glutaraldehyde is a valid and effective method for disinfecting bronchofibroscopes.
- Thorough washing followed by 10-minute immersion in phenolated glutaraldehyde ensures disinfection of instruments highly contaminated with S. marcescens and P. aeruginosa.