Jove
Visualize
Contact Us

Related Experiment Videos

TRACTOR, a program to locate subclones in a nucleotide sequence using only one sequencing reaction

J T Niemi1, P Mäntsälä

  • 1Department of Biochemistry and Food Chemistry, University of Turku, Finland.

Computer Applications in the Biosciences : CABIOS
|September 1, 1994
PubMed
Summary

The T-tracking method, utilizing the QuickBasic program TRACTOR, efficiently locates DNA clones within a known sequence using a single sequencing reaction. This approach significantly reduces workload in the final stages of DNA sequencing projects employing random subcloning strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expanding the scope of aromatic polyketides by combinatorial biosynthesis.

Combinatorial chemistry & high throughput screening·2003
Same author

The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures.

Molecular genetics and genomics : MGG·2001
Same author

A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin.

Molecular & general genetics : MGG·2000
Same author

Modifications of aclacinomycin T by aclacinomycin methyl esterase (RdmC) and aclacinomycin-10-hydroxylase (RdmB) from Streptomyces purpurascens.

Biochimica et biophysica acta·2000
Same author

Identification of a cyclase gene dictating the C-9 stereochemistry of anthracyclines from Streptomyces nogalater.

Antimicrobial agents and chemotherapy·2000
Same author

Crystal structure of Bacillus subtilis YabJ, a purine regulatory protein and member of the highly conserved YjgF family.

Proceedings of the National Academy of Sciences of the United States of America·1999
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • DNA sequencing projects often involve complex clone identification processes.
  • Random subcloning strategies can increase workload during the final stages of sequencing.
  • Efficient methods for locating clones are crucial for project completion.

Purpose of the Study:

  • To introduce the TRACTOR program for clone identification.
  • To demonstrate the utility of the T-tracking method in DNA sequencing.
  • To reduce the workload associated with random subcloning strategies.

Main Methods:

  • Development and application of the QuickBasic program TRACTOR.
  • Utilizing the T-tracking method for clone localization.
  • Integration with random subcloning strategies in sequencing projects.

Related Experiment Videos

Main Results:

  • The TRACTOR program successfully locates clones in known sequences.
  • The T-tracking method requires only one sequencing reaction per clone.
  • Significant reduction in end-stage project workload was observed.

Conclusions:

  • The TRACTOR program and T-tracking method offer an efficient solution for clone identification.
  • This approach streamlines the DNA sequencing workflow, particularly with random subcloning.
  • The T-tracking method is a valuable tool for reducing labor in genomics research.