Related Experiment Video
Updated: Aug 19, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Published on: July 28, 2016
(Z)-3-(4-bromophenyl)-3-(3-pyridyl)allylamine as substrate for studies of myeloperoxidase activity
S Lindvall1, G Rydell, L Johansson
1CNS, Preclinical R & D, Astra Arcus AB, Södertälje, Sweden.
Abstract:
(Z)-3-(4-Bromophenyl)-3-(3-pyridyl)allylamine (CPP 200) is transformed to the corresponding chloroimine by hypochlorite ion (ClO-) formed in the presence of myeloperoxidase. A scheme for this transformation is given. The influence of various compounds on this process has been studied. Cysteamine, cysteine and 6-chloro-3-hydrazino-pyridazine inhibited the transformation of CPP 200, while some p-hydroxyphenyl derivatives increased the rate of transformation of CPP 200. The increase seen on addition of the p-hydroxyphenyl derivatives is not a chloride-dependent reaction. Various mechanisms for the inhibiting effect as well as for the activating effect on the transformation of CPP 200 are discussed.
More Related Videos
Related Concept Videos
Regioselectivity of Electrophilic Additions-Peroxide Effect
ortho–para-Directing Activators: –CH3, –OH, –⁠NH2, –OCH3
Radical Oxidation of Allylic and Benzylic Alcohols
Radical Substitution: Allylic Bromination
Antiprotozoal Agents

