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Localization of the human genes encoding the two subunits of general transcription factor TFIIE
M Purrello1, C Di Pietro, A Rapisarda
1Istituto di Biologia generale, Università di Catania, Italy.
Genomics
|September 1, 1994
Abstract:
TFIIE is a general transcription factor for class II genes composed of two types of subunits, a large one of 56 kDa and a small of 34 kDa. By Southern analysis at high and at low stringency of a panel of mouse/human hybrid cell lines and by in situ chromosomal hybridization, we have demonstrated that both polypeptides are encoded by genes that are single copy in the human genome and are localized at 3q13-q21 and at 8p12, respectively. A TaqI RFLP (heterozygosity index of 0.07) was detected at the locus for the 56-kDa subunit.