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Cloning and chromosome localization of the mouse Ews gene
B Plougastel1, M G Mattei, G Thomas
1Laboratoire de Génétique des Tumeurs, INSERM CIF 9201, Paris, France.
Abstract:
The human EWS gene encodes a putative RNA binding protein. As a result of acquired chromosome rearrangement, the N-terminal portion of the EWS protein is fused to the DNA binding domain of either FLI-1 or ERG in the Ewing family of tumors and to the DNA binding domain of ATF1 in malignant melanoma of soft parts. We have determined the cDNA sequence of the mouse Ews gene. Its nucleotide sequence and its translation product demonstrate 93 and 98% homology with the human EWS cDNA and protein, respectively. The murine Ews locus lies within a conserved synteny segment between human chromosome 22q12 and mouse chromosome 11A1-A3.
Insights
Researchers sequenced the mouse Ews gene, finding high homology to the human EWS gene. This discovery aids in understanding EWS gene function in cancers like Ewing sarcoma.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- The human EWS gene encodes a protein involved in RNA binding.
- Chromosomal rearrangements involving the EWS gene are implicated in specific cancers, including Ewing sarcoma and malignant melanoma.
- These rearrangements fuse the EWS gene's N-terminal region to DNA-binding domains of other genes (FLI-1, ERG, ATF1).
Purpose of the Study:
- To determine the cDNA sequence of the mouse Ews gene.
- To compare the mouse Ews gene and protein sequence with its human counterpart.
- To investigate the chromosomal location of the murine Ews locus.
Main Methods:
- cDNA sequencing of the mouse Ews gene.
- Bioinformatic analysis for sequence homology comparison.
- Comparative genomic analysis to determine chromosomal location.
Main Results:
- The mouse Ews gene cDNA sequence was determined.
- The mouse Ews nucleotide sequence showed 93% homology to human EWS cDNA.
- The mouse Ews translation product demonstrated 98% homology to human EWS protein.
- The murine Ews locus was mapped to a conserved synteny segment on mouse chromosome 11A1-A3, corresponding to human chromosome 22q12.
Conclusions:
- The mouse Ews gene is highly homologous to the human EWS gene at both nucleotide and protein levels.
- The conserved synteny suggests functional importance of the EWS gene locus.
- The mouse model can be valuable for studying EWS gene function and related oncogenic mechanisms.