Antigenicity testing of inactivated oral enteric bacterial vaccines for human use

This study deals with the laboratory testing of the antigenicity of inactivated oral enteric bacterial vaccines for human use, viz. typhoid, dysentery, Escherichia coli and cholera vaccines. For the assays the following methods are applied : 1) various types of active mouse protection tests 2) serum and coproantibody determinations in immunized persons and experimental animals, 3) protective ability of the immune sera in mice and embryonated eggs, 4) hemolytic and bacteriolytic plaque assays. It is pointed out that the most important question: how far the results of these tests are in agreement with the degree of efficacy of vaccines in man, requires to be clarified.