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Improved large-scale purification of transducin, and its alpha and beta gamma subunits from frozen retinas
1Center for Biophysics and Computational Biology, University of Illinois, Urbana 61801.
Preparative Biochemistry
|November 1, 1994
Abstract:
The transducin heterotrimer and its alpha- and beta gamma-subunits have been purified from frozen bovine rod outer segments by modifying existing procedures. The methods described here are relatively simple and fast. The yield (ca. 8 mgs/100 retinas) and purity of the transducin heterotrimer and subunits from frozen retinas is equal to or larger than those previously obtained from fresh or frozen retinas.