Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

beta-Endorphin in human cerebrospinal fluid

W J Jeffcoate, L H Rees, L McLoughlin

    Lancet (London, England)
    |July 15, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Treatment of acromegaly with the growth hormone-receptor antagonist pegvisomant.

    The New England journal of medicine·2000
    Same author

    Hypothalamo-pituitary abnormalities in adult patients with langerhans cell histiocytosis: clinical, endocrinological, and radiological features and response to treatment.

    The Journal of clinical endocrinology and metabolism·2000
    Same author

    Complications of inferior petrosel sinus sampling.

    The Journal of clinical endocrinology and metabolism·2000
    Same author

    'Gangliocytomas' of the pituitary: a heterogeneous group of lesions with differing histogenesis.

    The American journal of surgical pathology·2000
    Same author

    Primary hyperaldosteronism (Conn syndrome): MR imaging findings.

    Radiology·2000
    Same author

    Screening children at risk of developing inherited endocrine neoplasia syndromes.

    Clinical endocrinology·2000

    Researchers developed a radioimmunoassay to measure beta-endorphin in human plasma and cerebrospinal fluid (CSF). Beta-endorphin was found in CSF at higher concentrations than in plasma, suggesting brain synthesis.

    Area of Science:

    • Neuroscience
    • Endocrinology
    • Biochemistry

    Background:

    • Beta-endorphin is a brain peptide with morphine-like activity.
    • It is structurally related to beta-lipotrophin (beta-L.P.H.), an anterior pituitary hormone.
    • Existing methods lacked specificity for differentiating beta-endorphin from beta-L.P.H.

    Purpose of the Study:

    • To develop and validate a radioimmunoassay for quantifying human beta-endorphin in plasma and cerebrospinal fluid (CSF).
    • To investigate the relationship between beta-endorphin levels in plasma and CSF.
    • To explore the potential site of beta-endorphin synthesis (brain vs. pituitary).

    Main Methods:

    • Development of a dual radioimmunoassay system utilizing two antisera to distinguish beta-endorphin from beta-lipotrophin (beta-L.P.H.).

    Related Experiment Videos

  • Measurement of beta-endorphin and beta-L.P.H. concentrations in human plasma and CSF samples.
  • Application of gel chromatography in conjunction with immunoassays for precise quantification.

    • Main Results:

    • A reliable radioimmunoassay for human beta-endorphin in plasma and CSF was established.
    • Beta-endorphin was detected in all 20 CSF samples tested.
    • CSF beta-endorphin concentrations were consistently higher than plasma levels, with no direct correlation.
    • Beta-endorphin was present in the CSF of hypopituitary patients with undetectable plasma beta-endorphin.

    Conclusions:

    • The developed radioimmunoassay allows for specific measurement of beta-endorphin in human biofluids.
    • Beta-endorphin levels in CSF are significantly higher than in plasma.
    • Findings suggest that beta-endorphin is synthesized within the brain, independent of the pituitary gland.