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Related Experiment Videos

Selective adhesion of hepatocytes on patterned surfaces

S N Bhatia1, M Toner, R G Tompkins

  • 1Surgical Services, Massachusetts General Hospital, Shriners Burns Institute, Cambridge 02139.

Annals of the New York Academy of Sciences
|November 30, 1994
PubMed
Summary

Researchers developed micropatterning techniques for stable hepatocellular function in bioartificial liver devices. This method uses selective hepatocyte adhesion on patterned surfaces, creating efficient nutrient transport channels for improved liver support.

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Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Bioengineering

Background:

  • Developing bioartificial liver devices requires stable hepatocellular function and efficient nutrient/waste transport.
  • Micropatterning technology offers a solution by creating alternating cell and transport channels.

Purpose of the Study:

  • To develop techniques for selective hepatocyte adhesion on patterned substrates.
  • To optimize hepatocyte patterning for bioartificial liver device applications.
  • To determine critical design criteria for micropatterned bioartificial liver devices through mathematical modeling.

Main Methods:

  • Utilized hydrophilic adhesive (AS) and hydrophobic nonadhesive (NAS) surfaces for selective cell adhesion.
  • Developed a spin-coating technique with collagen type I to pattern hepatocytes.

Related Experiment Videos

  • Assessed hepatocyte morphology and function in patterned and non-patterned configurations.
  • Employed mathematical modeling to simulate oxygen distribution and pressure drop in microchannels.

    • Main Results:

    • Achieved reproducible, selective hepatocyte adhesion on patterned glass substrates.
    • Demonstrated that patterned hepatocytes exhibit morphology and function comparable to stable sandwich cultures.
    • Identified optimal microchannel dimensions (0.6 cm length, 100 µm width, 10 µm height) and flow rate (2.0 x 10⁻⁶ mL/s) for efficient transport.

    Conclusions:

    • Micropatterning provides a viable strategy for creating stable hepatocellular function in bioartificial liver devices.
    • The developed techniques enable precise control over cell adhesion and arrangement.
    • Mathematical modeling is crucial for optimizing device design and ensuring in vivo compatibility.