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L cell clone developing plaques upon infection with measles virus (Edmonston strain)

H Sakae1, H Yoshikura, Y Yanagi

  • 1Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.

Archives of Virology
|January 1, 1994
PubMed

Insights

Researchers isolated measles virus-infected L cells that formed plaques. The plaque-forming and non-plaque-forming cells showed similar sensitivity to the virus, suggesting different host cell regulations for cytopathogenicity and infection sensitivity.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Measles virus (Edmonston strain) infection can induce cytopathic effects in host cells.
  • Understanding host cell responses to viral infection is crucial for developing antiviral strategies.

Purpose of the Study:

  • To investigate the characteristics of L cell clones that form cytopathic plaques upon measles virus infection.
  • To compare the sensitivity of plaque-forming and non-plaque-forming L cell clones to measles virus infection.

Main Methods:

  • Isolation of a specific L cell clone exhibiting cytopathic plaque formation after measles virus (Edmonston strain) infection.
  • Utilizing a newly developed UV-Vero assay to measure viral sensitivity in different L cell clones.

Main Results:

  • A distinct L cell clone capable of developing cytopathic plaques was successfully obtained.
  • No significant difference in measles virus sensitivity was observed between plaque-forming and non-plaque-forming L cell clones, as determined by the UV-Vero assay.

Conclusions:

  • Cytopathogenicity and sensitivity to measles virus infection are regulated by distinct host cell mechanisms.
  • Further research is needed to elucidate the specific molecular pathways involved in these differential regulations.

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