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L cell clone developing plaques upon infection with measles virus (Edmonston strain)
H Sakae1, H Yoshikura, Y Yanagi
1Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
Archives of Virology
|January 1, 1994
Summary
Researchers isolated measles virus-infected L cells that formed plaques. The plaque-forming and non-plaque-forming cells showed similar sensitivity to the virus, suggesting different host cell regulations for cytopathogenicity and infection sensitivity.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Measles virus (Edmonston strain) infection can induce cytopathic effects in host cells.
- Understanding host cell responses to viral infection is crucial for developing antiviral strategies.
Purpose of the Study:
- To investigate the characteristics of L cell clones that form cytopathic plaques upon measles virus infection.
- To compare the sensitivity of plaque-forming and non-plaque-forming L cell clones to measles virus infection.
Main Methods:
- Isolation of a specific L cell clone exhibiting cytopathic plaque formation after measles virus (Edmonston strain) infection.
- Utilizing a newly developed UV-Vero assay to measure viral sensitivity in different L cell clones.
Main Results:
- A distinct L cell clone capable of developing cytopathic plaques was successfully obtained.
- No significant difference in measles virus sensitivity was observed between plaque-forming and non-plaque-forming L cell clones, as determined by the UV-Vero assay.
Conclusions:
- Cytopathogenicity and sensitivity to measles virus infection are regulated by distinct host cell mechanisms.
- Further research is needed to elucidate the specific molecular pathways involved in these differential regulations.