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Related Experiment Videos

A versatile and general prokaryotic expression vector, pLACT7

S Chong1, G A Garcia

  • 1College of Pharmacy, University of Michigan, Ann Arbor 48109-1065.

Biotechniques
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

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Researchers developed a versatile plasmid, pLACT7, for controllable expression of tRNA-guanine transglycosylase (TGT) in E. coli. This new system offers high copy number and DNA yield, improving protein expression. Keywords: TGT, E. coli, plasmid, protein expression.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Microbiology

Context:

  • Previous work demonstrated constitutive overexpression of tRNA-guanine transglycosylase (TGT) using plasmid pTGT1 in E. coli.
  • The limitations of pET21b for TGT expression include low copy number, poor single-stranded DNA yield, and dependence on specific E. coli strains.
  • A need exists for a more versatile and efficient expression system for TGT.

Purpose:

  • To construct a controllable and versatile expression system for tRNA-guanine transglycosylase (TGT).
  • To combine advantageous features of pTZ18U and pET21b plasmids.
  • To develop a system suitable for a wide range of E. coli strains.

Summary:

  • A novel plasmid, pLACT7, was engineered by integrating features from pTZ18U and pET21b.

Related Experiment Videos

  • pLACT7 exhibits a high copy number and high yield of single-stranded DNA.
  • This plasmid incorporates both T7 and lac promoters for inducible protein expression in diverse E. coli strains.

    • Impact:

    • The pLACT7 plasmid provides a robust platform for controllable TGT expression.
    • This system enhances the efficiency and applicability of TGT research and biotechnological applications.
    • Facilitates wider use of TGT in various E. coli expression systems.