Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

"Checkerboard" DNA-DNA hybridization

S S Socransky1, C Smith, L Martin

  • 1Forsyth Dental Center, Boston, MA.

Biotechniques
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Underscoring long-term host-microbiome interactions in a physiologically relevant gingival tissue model.

NPJ biofilms and microbiomes·2025
Same author

A physiologically relevant culture platform for long-term studies of in vitro gingival tissue.

Acta biomaterialia·2023
Same author

The oral microbiome of patients undergoing treatment for severe aplastic anemia: a pilot study.

Annals of hematology·2019
Same author

Clinical and microbiological parameters of naturally occurring periodontitis in the non-human primate <i>Macaca mulatta</i>.

Journal of oral microbiology·2018
Same author

Impact of Oral Hygiene Discontinuation on Supragingival and Salivary Microbiomes.

JDR clinical and translational research·2018
Same author

Ligature-associated bacterial profiles are linked to type 2 diabetes mellitus in a rat model and influenced by antibody treatment against TNF-α or RAGE.

Clinical and experimental dental research·2017
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

This study presents a novel hybridization method for simultaneously analyzing multiple DNA samples and probes on a single membrane. This technique efficiently detects multiple bacterial species in various samples, including dental plaque.

Area of Science:

  • Molecular Biology
  • Genomics
  • Microbiology

Background:

  • High-throughput DNA analysis requires efficient hybridization techniques.
  • Current methods can be time-consuming and resource-intensive for large-scale sample analysis.
  • Detecting multiple microbial species in complex samples necessitates versatile detection platforms.

Purpose of the Study:

  • To develop and validate a novel, high-throughput method for DNA-DNA hybridization.
  • To enable simultaneous hybridization of numerous DNA samples with numerous DNA probes on a single membrane.
  • To assess the method's utility for detecting multiple bacterial species in clinical and environmental samples.

Main Methods:

  • Utilized a Miniblotter 45 device for fixing up to 43 denatured DNA samples in separate lanes on a membrane.

Related Experiment Videos

  • Performed 90-degree rotated, simultaneous hybridization with 43 different DNA probes on the same membrane.
  • Employed a MiniSlot device for aspirating bacterial cell lysates through membranes, creating horizontal lanes for hybridization with vertical probes (digoxigenin-labeled genomic or alkaline phosphatase-conjugated 16S rRNA probes).

    • Main Results:

    • Successfully demonstrated simultaneous hybridization of multiple DNA samples against multiple DNA probes on a single membrane.
    • Validated the method for analyzing bacterial cell lysates, enabling detection of multiple species.
    • Achieved simultaneous determination of multiple bacterial species presence in dental plaque samples.

    Conclusions:

    • The developed hybridization method offers a highly efficient approach for large-scale DNA analysis.
    • This technique facilitates the simultaneous detection of multiple bacterial species in single or multiple samples.
    • The method shows significant potential for applications in clinical diagnostics and environmental monitoring.