An immunodetection method for the quantitation of human acyl CoA:glycine N-acyltransferase in biological samples

An immunochemical method for quantitation of human acyl CoA:glycine N-acyltransferase (ACGNAT) has been developed. A 33 kD ovine ACGNAT was purified to homogeneity and was used to raise polyclonal antibodies in rabbits. These antibodies were then employed for developing a sensitive immunodiagnostic method as they cross-reacted with human ACGNAT. Using this methodology, a linear correlation was seen between specific activity and ACGNAT concentration in an ammonium sulphate precipitation fraction (40-60%) of hepatic tissues of children and adults of 4 h age to 11 year old (r = 0.99, n = 6).