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Related Experiment Videos

Solid tumor cells express functional "tethered ligand" thrombin receptor

M Z Wojtukiewicz1, D G Tang, E Ben-Josef

  • 1Department of Radiation Oncology, Wayne State University, Detroit, Michigan 48202.

Cancer Research
|February 1, 1995
PubMed
Summary
This summary is machine-generated.

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This study shows that thrombin receptors on tumor cells mediate alpha-thrombin

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Previous studies indicated alpha-thrombin enhances tumor cell metastasis.
  • The role of the thrombin receptor in mediating these effects was unclear.

Purpose of the Study:

  • To investigate if thrombin's effects on tumor cells are mediated by the thrombin receptor.
  • To confirm the presence and functionality of thrombin receptors on tumor cells.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) and Southern hybridization to detect thrombin receptor mRNA.
  • Immunoblotting using a specific antibody (mAb ATAP138) to detect thrombin receptor protein.
  • Peptide stimulation assays to assess tumor cell adhesion.

Main Results:

Related Experiment Videos

  • Thrombin receptor mRNA and protein were detected in human, rat, and mouse tumor cells.
  • The antibody's reactivity was blocked by a specific peptide (TRP 14), confirming specificity.
  • Peptides mimicking thrombin (TRP 14 and TRP 7) stimulated tumor cell adhesion to fibronectin.

Conclusions:

  • Solid tumor cells express functional thrombin receptors.
  • These receptors mediate alpha-thrombin's effects on tumor cell adhesion and potentially metastasis.