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Do trehalose and dimethyl sulfoxide affect intermembrane forces?

F Pincet1, E Perez, J Wolfe

  • 1Laboratoire de Physique Statistique de l'Ecole Normale Supérieure, universités Paris VI, France.

Cryobiology
|December 1, 1994
PubMed
Summary
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Trehalose and sorbitol do not significantly alter membrane interactions, suggesting their protective effects against dehydration are not due to direct bilayer repulsion. Dimethyl sulfoxide showed minor effects but sometimes destabilized membranes.

Area of Science:

  • Biophysics
  • Materials Science
  • Biochemistry

Background:

  • Trehalose and dimethyl sulfoxide (DMSO) are used to protect biological membranes during dehydration and cryopreservation.
  • These protectants are thought to preserve membrane integrity through various mechanisms, including solute exclusion and membrane stabilization.

Purpose of the Study:

  • To investigate the direct effects of trehalose, sorbitol, and DMSO on the interaction forces between lipid bilayers.
  • To understand the role of these solutes in membrane protection during dehydration and cryopreservation.

Main Methods:

  • Utilized a surface forces apparatus to measure short-range repulsive forces between phosphatidylcholine bilayers.
  • Quantified solute partitioning using gravimetric measurements.
  • Observed bilayer behavior in the presence of different solute concentrations.

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Main Results:

  • Trehalose and sorbitol, at high concentrations, did not significantly alter the repulsive forces between bilayers.
  • A slight increase in adhesion was observed with trehalose and sorbitol, attributed to osmotic effects.
  • Dimethyl sulfoxide showed minimal impact on bilayer interactions but occasionally caused bilayer perturbation and fusion.

Conclusions:

  • Trehalose and sorbitol likely protect membranes via mechanisms other than direct modulation of bilayer-bilayer repulsion.
  • Their protective effects may be primarily osmotic or related to water replacement.
  • Dimethyl sulfoxide's interaction with membranes is complex and can lead to instability.