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The NssBF element, a sequence of the Drosophila melanogaster retrotransposon 1731 potentially implicated in
J Lacoste1, F Fourcade-Peronnet
1UA CNRS 1135, Université P. et M. Curie, Paris, France.
Abstract:
The nuclear single-stranded DNA binding factor (NssBF) has been characterized as a nuclear protein that binds to a 26 nucleotides sequence in the long terminal repeat (LTR) of the Drosophila melanogaster 1731 retrotransposon. This sequence, called NssBF element, was analysed by gel retardation experiments using wild-type and mutated oligonucleotides. In vitro transcription experiments were performed and suggest that NssBF element binding protein(s) represses transcription through the 1731 promoter. Furthermore, computer assisted sequence comparisons put forward a possible role of this element and/or its associated DNA binding proteins in replication.