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Related Experiment Videos

Polycyclic aromatic hydrocarbons decrease intracellular glutathione levels in the A20.1 murine B cell lymphoma

J W Yuan1, J A Krieger, K R Maples

  • 1University of New Mexico College of Pharmacy, Toxicology Program, Albuquerque 87131.

Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology
|October 1, 1994
PubMed
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Polycyclic aromatic hydrocarbons (PAHs) cause a temporary reduction in intracellular glutathione (GSH) levels in lymphocytes. This depletion of GSH may play a role in PAH-induced lymphocyte cell injury.

Area of Science:

  • Immunotoxicology
  • Cellular Biochemistry
  • Environmental Health

Background:

  • Polycyclic aromatic hydrocarbons (PAHs) are known to inhibit lymphocyte activation and disrupt intracellular calcium (Ca2+) homeostasis.
  • Both chemical oxidants and ionizing radiation can increase intracellular Ca2+ in lymphocytes.
  • Oxidative stress in cells is characterized by increased intracellular Ca2+ and decreased intracellular glutathione (GSH).

Purpose of the Study:

  • To investigate whether PAHs affect intracellular glutathione (GSH) levels in lymphocytes.
  • To determine the specific PAHs that impact GSH levels and the extent of this impact.

Main Methods:

  • Utilized established glutathione assays to quantify intracellular GSH levels.
  • Exposed A20.1 murine B lymphoma cells to various PAHs.

Related Experiment Videos

  • Measured changes in reduced GSH concentrations over a 2-4 hour period.

    • Main Results:

    • PAHs induced a transient decrease in intracellular GSH levels in A20.1 cells.
    • Benzo(a)pyrene, 7,12-dimethylbenz(a)anthracene, benz(a)anthracene, and anthracene caused a 10-25% reduction in reduced GSH.
    • Benzo(e)pyrene did not significantly alter intracellular GSH levels.

    Conclusions:

    • PAHs can deplete intracellular glutathione (GSH) in lymphocytes.
    • Glutathione depletion is a potential mechanism contributing to cell injury in lymphocytes exposed to PAHs.