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Related Experiment Videos

Cloning of a cDNA encoding murine tetranectin

C B Sørensen1, L Berglund, T E Petersen

  • 1Protein Chemistry Laboratory, University of Aarhus, Denmark.

Gene
|January 23, 1995
PubMed
Summary

Researchers cloned murine tetranectin (TN) cDNA, revealing a 202 amino acid protein highly conserved with human TN. Mouse TN expression is highest in lung and skeletal muscle tissues.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Tetranectin (TN) is a protein with known biological functions.
  • Understanding the genetic and expression profiles of TN is crucial for its study.

Purpose of the Study:

  • To isolate and characterize the full-length cDNA encoding murine tetranectin (TN).
  • To determine the nucleotide sequence and deduce the amino acid sequence of mouse TN.
  • To investigate the evolutionary conservation and tissue-specific expression of murine TN.

Main Methods:

  • Isolation and cloning of full-length cDNA from a murine lung lambda ZAPII cDNA library.
  • Determination of the complete nucleotide sequence.
  • Deduction of the amino acid sequence and signal peptide length.
  • Comparative analysis of amino acid identity with human TN.
  • Analysis of tissue-specific gene expression.

Main Results:

  • A full-length cDNA encoding murine tetranectin (TN) was successfully isolated and cloned.
  • The deduced protein sequence consists of 202 amino acids, including a 21 amino acid signal peptide.
  • Murine TN shares a 79% amino acid identity with human TN, indicating high evolutionary conservation.
  • The highest expression levels of mouse TN were detected in lung and skeletal muscle tissues.

Conclusions:

  • The successful cloning and sequencing of murine tetranectin provide a foundation for further functional studies.
  • The high evolutionary conservation suggests an important conserved role for tetranectin across species.
  • Tissue-specific expression patterns highlight potential roles of TN in lung and skeletal muscle physiology.

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