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Sequence of the mouse adenovirus serotype-1 DNA encoding the precursor to capsid protein VI
B Song1, K R Spindler, C S Young
1Department of Microbiology, Columbia University, New York, NY 10032.
Abstract:
The nucleotide sequence predicted to encode the precursor to virion structural protein VI (preVI) of mouse adenovirus (Ad) serotype-1 (MAV-1) was determined. The 237-amino-acid sequence has 45% identity and 66% similarity to the human Ad serotype-2 preVI sequence. There is a marked conservation at the C terminus, the last eleven residues of which may be necessary for activating the Ad endoproteinase, and at the N terminus, including the consensus endoproteinase cleavage site.
Insights
Researchers determined the mouse adenovirus (MAV-1) protein VI precursor (preVI) sequence, finding significant similarity to human adenovirus preVI. This conservation suggests a shared functional role in viral replication and endoproteinase activation.
Area of Science:
- Virology
- Molecular Biology
- Structural Biology
Background:
- Adenoviruses are non-enveloped viruses with icosahedral capsids, responsible for various infections in mammals.
- Structural protein VI (pVI) plays a crucial role in the viral life cycle, including cell entry and DNA release.
- Mouse adenovirus (MAV-1) is a significant pathogen in laboratory mice, necessitating research into its molecular mechanisms.
Purpose of the Study:
- To determine the nucleotide sequence encoding the precursor to virion structural protein VI (preVI) of mouse adenovirus (Ad) serotype-1 (MAV-1).
- To compare the MAV-1 preVI sequence with homologous sequences from other adenoviruses, particularly human Ad serotype-2.
- To identify conserved regions and potential functional domains within the MAV-1 preVI sequence.
Main Methods:
- Bioinformatic analysis of the MAV-1 genome to identify the gene encoding preVI.
- Amino acid sequence prediction based on the determined nucleotide sequence.
- Sequence alignment and comparison with the human Ad serotype-2 preVI sequence using established algorithms.
Main Results:
- The nucleotide sequence encoding MAV-1 preVI was determined, predicting a 237-amino-acid precursor protein.
- The MAV-1 preVI sequence exhibits 45% identity and 66% similarity to the human Ad serotype-2 preVI sequence.
- Significant sequence conservation was observed at both the N terminus (including the endoproteinase cleavage site) and the C terminus (potentially involved in endoproteinase activation).
Conclusions:
- The MAV-1 preVI sequence shares significant homology with human adenovirus preVI, indicating conserved structural and functional properties.
- The conserved C-terminal residues may be critical for activating the adenovirus endoproteinase, a key enzyme in viral replication.
- The conserved N-terminal region, including the cleavage site, suggests a conserved mechanism for processing and activating protein VI during viral assembly or infection.