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Related Experiment Videos

Sry is a transcriptional activator

R A Dubin1, H Ostrer

  • 1Department of Pediatrics, New York University Medical Center, New York 10016.

Molecular Endocrinology (Baltimore, Md.)
|September 1, 1994
PubMed
Summary

The SRY gene initiates testis development. Mouse Sry protein binds DNA and activates transcription, while human SRY

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Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • The SRY gene is a crucial genetic switch for testis determination.
  • Human and mouse SRY proteins share a DNA-binding HMG-box domain but little else.
  • Both human and mouse SRY proteins are localized to the nucleus.

Purpose of the Study:

  • To investigate the DNA-binding and transcriptional activation properties of mouse Sry.
  • To compare the functional domains of mouse Sry and human SRY.
  • To identify the specific regions responsible for Sry's transcriptional activation.

Main Methods:

  • In vitro DNA-binding assays using oligonucleotide pools.
  • Reporter gene assays with heterologous promoters and GAL4/LexA systems.
  • Mapping of functional domains through protein fusion constructs.

Main Results:

  • Mouse Sry's HMG-box binds the AACAAT sequence with high affinity.
  • Mouse Sry activates transcription from reporter genes containing AACAAT sites.
  • Transcriptional activation function of mouse Sry maps to a glutamine/histidine-rich domain.
  • Human SRY did not demonstrate transcriptional activation in the GAL4 system.

Conclusions:

  • Both human and mouse SRY encode nuclear, DNA-binding proteins.
  • Mouse Sry functions as a transcriptional activator with distinct DNA-binding and activation domains.
  • Functional differences may exist between mouse Sry and human SRY in transcriptional activation.

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