Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TKO'ed: lox, stock and barrel

C A Chambers1

  • 1Department of Molecular and Cellular Biology, University of California at Berkeley 94720.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|December 1, 1994
PubMed
Summary

Researchers can now create specific gene mutations in mice at controlled times and locations. This powerful new method enhances gene targeting for studying complex gene functions in vivo.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chinese herbal medicine for atopic eczema.

The Cochrane database of systematic reviews·2005
Same author

Chinese herbal medicine for atopic eczema.

The Cochrane database of systematic reviews·2004
Same author

Topical tacrolimus for cutaneous lupus erythematosus.

The British journal of dermatology·2003
Same author

The expanding world of co-stimulation: the two-signal model revisited.

Trends in immunology·2001
Same author

CTLA-4-mediated inhibition in regulation of T cell responses: mechanisms and manipulation in tumor immunotherapy.

Annual review of immunology·2001
Same author

CTLA-4--the costimulatory molecule that doesn't: regulation of T-cell responses by inhibition.

Cold Spring Harbor symposia on quantitative biology·2001

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Homologous recombination enables the generation of mutant mice to study gene function in vivo.
  • Controlling mutation timing, tissue specificity, and mutation type is crucial for advanced genetic studies.

Purpose of the Study:

  • To demonstrate the utility of Cre-lox site-specific recombination in conjunction with homologous recombination for generating conditional mutations in vivo.
  • To enhance flexibility in gene targeting strategies for in vivo studies.

Main Methods:

  • Utilizing the Cre-lox conservative site-specific recombination system from bacteriophage P1.
  • Combining Cre-lox technology with established homologous recombination techniques.
  • Generating temporal- and cell-restricted mutations in mice.

Main Results:

  • Successful application of Cre-lox recombination for conditional gene targeting in vivo.
  • Demonstrated ability to control the developmental stage and tissue-specific nature of mutations.
  • Enhanced flexibility in creating complex gene modifications.

Conclusions:

  • The combination of Cre-lox and homologous recombination offers a powerful tool for generating precisely controlled mutations in vivo.
  • This technical advance significantly impacts the study of complex gene functions by allowing greater flexibility in gene targeting.
  • Future research can leverage this method for more sophisticated investigations into gene roles in biological systems.

Related Experiment Videos