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Characterization of a 43 kD protein associated to aminopeptidase A from murine kidney
S Mentzel1, E P de Leeuw, J P van Son
1Department of Pathology, University Hospital Nijmegen, The Netherlands.
Biological Chemistry Hoppe-Seyler
|September 1, 1994
Abstract:
SDS-PAGE of affinity-purified APA under reducing conditions showed in addition to the specific APA band of M(r) 130 kD, a second band of M(r) 43 kD. Internal amino acid sequencing of three tryptic peptides from this second band, that was cut out of the polyacrylamide gel, matched the actin sequence. The identity of the 43 kD band was also confirmed by Western blotting.