Polymorphic DdeI restriction sites in mitochondrial d-Loop DNA from Emilian blood donors

We describe two new polymorphic sites identified by DdeI digestion of human mitochondrial D-Loop DNA. Their frequencies were estimated on a random group of 54 unrelated blood donors, born and descending from female ancestors of the Province of Reggio Emilia (Central Emilia, Northern Italy). These newly detected polymorphisms were generated by A to G transitions at positions 16300 and 16482 of mt-DNA. They were found at a 5% frequency in our sample whereas the remaining 95% of the sample exhibited a Cambridge sequence restriction pattern.