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Controlled release systems for proteins based on gelatin microspheres

J K Rao1, D V Ramesh, K P Rao

  • 1Biomaterials Laboratory, Central Leather Research Institute, Adyar, Madras, India.

Journal of Biomaterials Science. Polymer Edition
|January 1, 1994
PubMed
Summary
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Biodegradable gelatin microspheres were developed for controlled protein release. These microspheres effectively encapsulated bovine serum albumin (BSA), showing sustained release over 8 days without degradation.

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Polymer Chemistry

Background:

  • Biodegradable polymers are crucial for controlled drug delivery.
  • Gelatin microspheres offer potential for encapsulating therapeutic proteins.
  • Controlled release systems are needed for peptides and proteins.

Purpose of the Study:

  • To prepare and characterize biodegradable gelatin microspheres.
  • To investigate the in vitro controlled release of bovine serum albumin (BSA).
  • To assess the stability of BSA within the microspheres.

Main Methods:

  • Gelatin microspheres were prepared using a polymer dispersion technique.
  • Bovine serum albumin (BSA) was labeled with fluorescein isothiocyanate (FITC) for quantification.

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  • Microsphere morphology was analyzed using optical and scanning electron microscopy (SEM).
  • In vitro release studies and SDS-PAGE were performed.

    • Main Results:

    • Gelatin microspheres exhibited a solid spherical morphology.
    • Entrapment efficiency for FITC-BSA was approximately 62%.
    • In vitro release showed 51% release on day 1, followed by zero-order kinetics, with total release over 8 days.
    • SDS-PAGE confirmed no degradation of BSA.

    Conclusions:

    • Biodegradable gelatin microspheres are suitable for the controlled release of proteins like BSA.
    • The developed method provides efficient entrapment and sustained release.
    • BSA integrity is maintained during the microsphere preparation and release process.