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Cloning and sequencing a putative pyrophosphate-dependent phosphofructokinase gene from Entamoeba histolytica
M Huang1, R A Albach, K P Chang
1Department of Microbiology/Immunology, Chicago Medical School, IL 60064.
Biochimica Et Biophysica Acta
|January 25, 1995
Abstract:
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) gene from Entamoeba histolytica was cloned from its genomic library and sequenced. The open reading frame has 1149 bp and codes for a protein of 41.5 kDa. The deduced amino acid sequence of E. histolytica PPi-PFK has 25 to 28% identity to the PPi-PFKs from Propionibacterium freudenreichii, Naegleria fowleri and potato. The amino acid residues known to contribute to the active site of PPi-PFK from P. freudenreichii are conserved.