Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Injectable collagen as a pH-sensitive hydrogel

J Rosenblatt1, B Devereux, D G Wallace

  • 1Collagen Corporation, Palo Alto, CA 94303.

Biomaterials
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of open field movement organization and spatial orientation in 5xFAD mice.

Brain research·2026
Same author

Longitudinal evaluation of open field and string-pulling behaviors in mice.

Behavioural brain research·2025
Same author

Growth velocity of fetal sacrococcygeal teratoma as predictor of perinatal morbidity and mortality: multicenter study.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2024
Same author

Application of scaling to mouse spontaneous movement: Path curvature varies with speed and linear distance features isochrony.

Behavioural brain research·2024
Same author

Non-invasive cell-free DNA prenatal screening for trisomy 21 as part of primary screening strategy in twin pregnancy.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2023
Same author

Impact of prenatal estimation of the risk of respiratory distress in neonates with congenital pulmonary malformations on the choice of delivery site.

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2023

Injectable collagen fiber properties change with pH and temperature. Higher pH increases fiber rigidity and attraction, while higher temperatures enhance hydrophobic forces, altering gel characteristics.

Area of Science:

  • Biomaterials Science
  • Colloid and Surface Chemistry
  • Biophysics

Background:

  • Injectable collagen is a dispersion of collagen fibers in an aqueous solution.
  • Collagen fiber structure and properties depend on electrostatic and hydrophobic forces between collagen molecules.
  • Understanding these forces is crucial for developing collagen-based biomaterials.

Purpose of the Study:

  • To investigate the impact of pH and temperature on the structure and mechanical properties of injectable collagen fiber dispersions.
  • To elucidate the role of electrostatic and hydrophobic interactions in collagen fiber behavior.
  • To characterize the differences in gel properties formed under varying conditions.

Main Methods:

  • Dynamic rheological measurements were employed to assess mechanical properties.

Related Experiment Videos

  • Polarized microscopy was used to visualize collagen fiber structure.
  • Experiments were conducted across a pH range of 6 to 9 and temperatures from 283 K to 298 K.

    • Main Results:

    • Increased pH led to more rigid collagen fibers and stronger inter-fiber attractive forces, attributed to enhanced net-electrostatic attractive forces from amino acid side chain ionization.
    • Elevated temperatures resulted in fiber rigidification via strengthened hydrophobic attractive forces.
    • Gels formed at lower pH and higher temperatures exhibited distinct properties compared to those formed at higher pH and lower temperatures.

    Conclusions:

    • Both pH and temperature significantly influence the structural and mechanical properties of injectable collagen dispersions.
    • Electrostatic forces play a key role at higher pH, while hydrophobic forces become more dominant at elevated temperatures.
    • The findings provide insights into controlling collagen gel properties for various applications.