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Related Experiment Videos

Bronchial provocation test with allergen: comparison between two different techniques

E Crimi1, A Balbo, M Lazzarotti

  • 1Dipartimento di Scienze Motorie, Università di Genova, Italy.

Monaldi Archives for Chest Disease = Archivio Monaldi Per Le Malattie Del Torace
|December 1, 1994
PubMed
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Bronchial provocation tests (BPTs) using cumulative allergen doses or a single dose yield comparable early and late asthmatic responses. However, methacholine sensitivity after allergen challenge was not reproducible with either BPT method.

Area of Science:

  • Allergy and Immunology
  • Pulmonary Medicine
  • Respiratory Research

Background:

  • Allergen bronchial provocation tests (BPTs) are crucial for assessing asthma treatment efficacy.
  • Standard BPTs typically involve determining the allergen dose causing a 20% fall in forced expiratory volume in one second (PD20) and administering this single dose on study days.
  • Allergen inhalation can trigger early asthmatic responses (EAR) or both EAR and late asthmatic responses (LAR).

Purpose of the Study:

  • To compare the efficacy of two BPT methods: cumulative increasing doses versus a single predetermined PD20 dose.
  • To evaluate if these different BPT methods yield comparable results for EAR, LAR, and methacholine (MCh) sensitivity.
  • To establish the reproducibility of allergen-induced changes in MCh sensitivity.

Main Methods:

Related Experiment Videos

  • A comparative study involving twelve asthma patients.
  • Two BPT methods were employed: one with increasing cumulative allergen doses up to PD20, and another with a single predetermined PD20 dose.
  • Comparison of early asthmatic response (EAR), late asthmatic response (LAR), and allergen-induced changes in methacholine (MCh) sensitivity.

Main Results:

  • Both the cumulative increasing dose and single dose BPT methods produced similar EAR and LAR.
  • The increasing dose method showed a trend towards more severe EAR compared to the single dose method.
  • The ratio of postallergen to preallergen MCh sensitivity demonstrated poor reproducibility across both methods.

Conclusions:

  • The choice between cumulative increasing allergen doses or a single PD20 dose in BPTs does not significantly alter the observed EAR and LAR.
  • Neither BPT method reliably predicts changes in MCh sensitivity following allergen challenge, indicating poor reproducibility.
  • Further research is needed to refine BPT methodologies for consistent assessment of airway hyperresponsiveness.