Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prolactin and the immune system

F Ferrag1, J J Lebrun, P Touraine

  • 1INSERM Unité 344, Faculté de Médecine Necker, Paris, France.

Immunomethods
|August 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A transcriptionally active pRb-E2F1-P/CAF signaling pathway is central to TGFβ-mediated apoptosis.

Cell death & disease·2012
Same author

Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse.

Experimental neurology·2008
Same author

Gene therapy for long-term restoration of circulating thymulin in thymectomized mice and rats.

Gene therapy·2006
Same author

Fibronectin receptor defects in NOD mouse leucocytes: possible consequences for type 1 diabetes.

Scandinavian journal of immunology·2004
Same author

A thymulin analogue peptide with powerful inhibitory effects on pain of neurogenic origin.

Neuroscience·2003
Same author

Zinc and immune function.

European journal of clinical nutrition·2002

Prolactin (PRL) receptors, including short and long forms, are present in lymphoid tissues across species. Prolactin receptor signaling involves JAK2 tyrosine kinase, crucial for cell mitogenesis.

Area of Science:

  • Immunology
  • Endocrinology
  • Molecular Biology

Background:

  • Prolactin (PRL) exerts diverse physiological effects, with a well-established immunoregulatory role.
  • The prolactin receptor (PRLR) exists in short and long isoforms, generated by alternative splicing, with variations across species.
  • While humans possess only the long PRLR form, rodents have both.

Purpose of the Study:

  • To investigate the presence and expression of prolactin receptor isoforms in lymphoid tissues of humans, mice, and rats.
  • To analyze the expression of prolactin transcripts in human and rat immune cells.
  • To elucidate the early events in prolactin receptor signal transduction, including the role of JAK2.

Main Methods:

  • Reverse transcriptase-polymerase chain reaction (RT-PCR) to detect PRL receptor and PRL transcripts.

Related Experiment Videos

  • Quantitative PCR (Q-PCR) to measure PRL receptor transcript levels in rat thymus and spleen.
  • Western blotting and immunoprecipitation to identify and study the association of JAK2 with the PRLR.

    • Main Results:

    • Both short and long PRL receptor transcripts were detected in lymphoid tissues of humans, mice, and rats, with variable ratios in rodents.
    • Prolactin transcripts were consistently found in human lymphocytes and occasionally in rat thymus.
    • The tyrosine kinase JAK2 was identified as constitutively associated with the PRLR, and its phosphorylation, along with PRLR dimerization, was shown to be an early event in PRL signaling.

    Conclusions:

    • Prolactin receptors and their isoforms are expressed in lymphoid tissues across species, suggesting conserved roles in immune function.
    • The signaling pathway of prolactin involves JAK2 and requires receptor dimerization and phosphorylation for initiating signal transduction.
    • Further research into PRL's immunoregulatory functions and its receptor signaling is warranted.