Recent studies of enzymically controlled steps in B12 biosynthesis
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Updated: Aug 13, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Published on: November 22, 2014
1Department of Chemistry, Texas A&M University, College Station 77843-3255.
Abstract:
The acquisition and sequencing of the genes encoding the enzymes for vitamin B12 biosynthesis in Salmonella typhimurium and Pseudomonas denitrificans has dramatically altered the direction of research on the pathway from uroporphyrinogen III to the corrinoids. Through a combination of molecular biology, organic chemistry and NMR spectroscopy, logical progression along the sequence is being made. Recent work from our laboratory is focused on the discovery and specificities of the methyltransferases connecting uroporphyrinogen III with cobyrinic acid, the temporal resolution of cobalt insertion and a comparison of the anaerobic pathway in S. typhimurium and the aerobic pathway in Ps. denitrificans. The implication of two parallel routes to corrins in these bacteria is discussed.
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