[Isolation, purification and the characteristics of 70 KD pronucleation glycoprotein in the bile]

In recent years, the great achievement in the etiology of cholelithiasis is the discovery of pro- and anti-nucleating factors in human bile. Using Con-A affinity chromatography combined with Sephadex G-200 molecular sieve technique, we have isolated and purified a strong pronucleating glycoprotein in gallbladder bile. Its molecular weight is 70KD, the quantity ratio of neutral hexose to protein in this glycoprotein is 15.88%. One molecule has about 640 amino acid residues. Its N-terminal amino acid sequence is N-Ser-His-Gly-Met-Arg-Gln-Tyr-Tyr-Met-Lys. The pathogenic and biological significance of this glycoprotein in the pathogenesis of gallstone were discussed.